The Antibody Characterization Laboratory (ACL) is the laboratory responsible for the development of well-characterized monoclonal antibody reagents. The NCI’s Office of Cancer Clinical Proteomics Research funds ACL as a resource to the entire cancer research community. ACL produces monoclonal antibodies to putative cancer targets selected by an Antibody Scientific Committee, and each antibody is characterized using optimized Standard Operating Procedures (SOPs). Approximately once a year there is a reagent target request open to the extramural research community. ACL is operated by Leidos Bi… [learn more]

The Antibody Engineering Program (AEP) is located at the Laboratory of Molecular Biology, which is part of the Center for Cancer Research (CCR), an intramural program at the National Cancer Institute (NCI). AEP focuses on generating therapeutic antibodies using phage display technology, including single-domain antibodies (also commonly known as ‘nanobodies’). Antibody-based therapeutics have emerged as a major component in clinical applications, and single-domain antibodies have shown promise in targeting difficult antigens and buried sites. Dr. Mitchell Ho and colleagues at the NCI have d… [learn more]

The Biopharmaceutical Development Program (BDP) provides resources for the development of investigational biological agents. The BDP supports feasibility through development and Phase I/II cGMP manufacturing plus regulatory documentation.The BDP was established in 1993. We support the development of monoclonal antibodies, recombinant proteins, therapeutic peptides, and DNA vaccines, virus therapeutics and vaccines, gene therapy products, and other biological agents. Since its inception, BDP has completed more than 100 projects. The program has released over 220 lots of different products since… [learn more]

The BRB Preclinical Biologics Repository is a central repository that supplies reagents to the broad research community. This NCI-sponsored repository is managed by NCI's Biological Resources Branch (BRB) in the Development Therapeutics Program (DTP) of the Division of Cancer Treatment and Diagnosis (DCTD). The BRB Preclinical Biologics Repository stores cytokines, monoclonal antibodies, and other biologic reagents under carefully controlled conditions to ensure a uniform supply of high-quality reagents. These reagents may be used for non-clinical research by qualified investigators … [learn more]

The CCR Collaborative Bioinformatics Resource (CCBR) is a centrally funded resource group which provides a mechanism for CCR researchers to obtain many different types of bioinformatics assistance to further their research goals. The group has expertise in a broad range of bioinformatics topics, and as such, its goal is to provide a simplified central access point for CCR researchers. Requests for any type of Bioinformatics support should be through the CCBR Project Submission Form. Types of Collaborative Expertise: Next Generation Sequence (NGS) data analysis (RNAseq, scRNAseq, ATAC-seq,… [learn more]

Radiation Oncology Branch is part of CCR. Bioinformatics core is a collaborative resource to support ROB branch and provide service to ROB investigators from NCI and other Institutes access to new technologies, bioinformatics, statistical analysis related to genetics/genomics, and offers access to in-house built software tools. Bioinformatics analysis: work with research scientists to provide consulting prior to experiments, analysis of high-throughput sequencing, gene expression, metabolomics, proteomics, and other biological data to identify: prognostic biomarkers, transcriptio… [learn more]

The Spatial Imaging Technology Resource (formerly the Nanoscale Protein Analysis Section of the Collaborative Protein Technology Resource or CPTR) provides expertise and service in state-of-the-art protein analysis technologies to advance CCR research in basic discovery and translation/clinical studies. Applications include: Quantitative profiling of cell signaling and post-translational modification, Measurement of cytokine, metabolite, and serum/plasma markers, Identification and validation of biomarkers & therapeutic targets, Assessment of on‐ … [learn more]

CCR VOLUME Electron Microscopy (CVEM) aims to develop new techniques in volume electron microscopy (vEM) and, in collaboration with CCR Investigators, apply these techniques to research cell biological questions well-suited for study by high-resolution 3D electron microscopy. CVEM, headed by Kedar Narayan, uses focused ion beam scanning electron microscopy (FIB-SEM), array tomography (AT), electron tomography (ET) and other complementary methods to visualize ultrastructural features of cells and tissues, including cell membranes, cellular organelles, and virus par… [learn more]

The Chemical Synthesis Group is a component of the Chemical Biology Laboratory. This facility provides synthetic chemistry resources and expertise to the NCI Intramural community. The facility’s capabilities include: Providing expertise, consultation, and experience in areas of chemical synthesis and purification., Providing custom synthesis and support for the development of small molecule tools or the synthesis of known ligands or probes which are not commercially available., Synthesis of materials for pre-clinical investigations., … [learn more]

Bruker AVANCE 400 and 500 MHz NMR instruments. Helium Cryoprobe technology on the 500 MHz machine for added sensitivity, especially for Carbon-13 spectra. Access to a second 500 MHz instrument with Prodigy Liquid Nitrogen-cooled cryoprobe. User Accounts can be created for automated setup and experiment acquisition.  Use of ICONNMR automated software with 24-slot sample changers on each machine. User training available. Some service to those who are unfamiliar with NMR can be performed either as a one-time aid for compound identification or as a collaborative project for more extensive NMR nee… [learn more]

The COP evaluates novel therapies in pet dogs with cancer to improve outcomes for human patients and established the Comparative Oncology Trial Consortium (COTC), a collaborative effort of NCI and extramural comparative oncology centers at 24 veterinary schools across the United States and Canada, with the goal to integrate naturally occurring cancer models into the development path of new cancer drugs for humans. The COP complements translational research efforts through the characterization and use of relevant and naturally occurring cancer models tha… [learn more]

The Developmental Therapeutics Program (DTP) within NCI's Division of Cancer Treatment and Diagnostics (DCTD), has maintained a low temperature repository of transplantable in vivo-derived tumors and in vitro-established tumor cell lines from various species. This Tumor Repository, located at the National Cancer Institute in Frederick serves as a resource for viable, pathogen-screened experimental tumor lines, many of which are not available elsewhere. The Repository makes these materials available to qualified investigators as a service to the cancer research community. Please find the ma… [learn more]

DTP maintains a repository of synthetic compounds and pure natural products that are available to investigators for non-clinical research purposes. The Repository collection is a uniquely diverse set of more than 200,000 compounds that have been either submitted to DTP for biological evaluation or, in some cases, synthesized under DTP auspices. Samples obtained from the NCI/DTP Open Chemicals Repository are available at no cost except for shipping charges. Importantly, investigators should note that chemical structures of these substances are those assigned by the… [learn more]

EIB Microscopy Facility has the following instruments: Abberior STEDYCON super-resolution (STED) microscope, Zeiss LSM 880-NLO confocal / two-photon system, Yokogawa CSU-W1 spinning disk confocal microscope, Zeiss AxioObserver Z1 wide-field microscope, As a multi-user facility, the different instruments provide a wide range of imaging modes for EIB scientists, from standard immunohistochemistry, through brightfield and wide-field epifluorescence imaging, to highly complex live cell confocal micro… [learn more]

The Clinical Flow Cytometry Laboratory provides extensive support for NCI clinical protocols by providing diagnostic testing for leukemia and lymphoma in patients either on NCI clinical protocols or undergoing testing to determine eligibility for NCI protocols. The Unit provides clinical flow cytometric evaluation of blood, bone marrow, fine needle aspirates, tissue biopsies and body fluids for hematolymphoid neoplasia in NCI and NIH patients. The majority of flow cytometric testing performed by the Unit involves detection of minimal residual disease (patients are status post-therapy at ou… [learn more]

The CCR Molecular Modeling Core, under the direction of Dr. Stewart Durell, was established to provide a convenient way for experimentalists to take advantage of molecular modeling and computational biology. In the framework of consultations, scientists can explore how such tools could benefit their projects. The Core then acts as a clearinghouse to help set up and use appropriate software, and/or form collaborations with other scientists. Upon mutual agreement, Core personnel will enter into a collaboration to do the modeling work themselves. All services of the Core are free … [learn more]

The Molecular Pathology Unit is intended to spearhead opportunities for bridging basic and clinical research efforts by more precisely optimizing the development, characterization and utilization of models of human disease. The initiative approach includes both applied research and collaborations aimed at developing new reagents, methods, and technologies in preclinical diagnostic medicine; thus enhancing capabilities to integrate molecular and systemic aspects of disease mechanisms. Designing and employing state of the art medical and pathology investigative tools to the study of animal model… [learn more]

Thousands of molecular targets have been measured in the NCI panel of 60 human tumor cell lines. Measurements include protein levels, RNA measurements, mutation status and enzyme activity levels. You can choose to search for a target of interest, or you may browse through a list of targets. Follow the links for a target to retrieve the 60 cell line data (either text or graphical), to run COMPARE (find Targets or Compounds whose patterns correlate with a Target of interest) and to link to various databases with information (function, sequences, disease associations) about the target. To co… [learn more]

The Molecular Targets Program (MTP) is an organizational entity within the Center for Cancer Research (CCR) at NCI. The MTP provides the focus and infrastructure to enable CCR tenured and tenure-track Principal Investigators to initiate and pursue interdisciplinary, applied, collaborative, molecularly targeted drug discovery research within a matrix organizational format that is both supportive of and complementary to the traditional NCI/NIH intramural Lab/Branch organization. The MTP mission statement further defines the new organizational model. The initi… [learn more]

NCI established the Nanotechnology Characterization Laboratory (NCL) to support the extramural research community to accelerate the progress of nanomedicine by providing preclinical characterization and safety testing of nanoparticles. It is a collaborative effort between NCI, the US Food and Drug Administration (FDA), and the National Institute of Standards and Technology (NIST). NCL performs: Characterization of strategies with proven efficacy for cancer therapy, diagnosis, or vaccines, Evaluation of novel, early-stage nanomedicine platforms with potential… [learn more]

The Central Repository provides the research community at the NCI with various cryogenic services, including low-temperature storage from +4° C to -196° C, controlled-rate freezing, computerized inventory and distribution of samples. The Central Repository is operated by Leidos Biomedical Research Inc. on behalf of NCI as part of the Frederick National Laboratory. Services may be provided to other NIH Institutes for specific projects upon request. Contacting This Facility: To contact this facility, please refer to the contact details below. Please do not send your request via CREx.: … [learn more]

The goals of the Bioinformatics Training and Education Program within NCI/CCR are: To make researchers aware of the bioinformatics resources available to them, To provide training and guidance on these resources regularly and at the appropriate level for the researcher throughout their training or tenure, To enable them to perform as much of the bioinformatics analyses of their data as they wish to learn, [learn more]

We are a bioinformatics team within the Center for Biomedical Informatics and Information Technology’s (CBIIT’s) Cancer Informatics Branch (CIB)—soon to be referred to as the Informatics and Data Science (IDS) Program. Headed by our chief, Dr. Daoud Meerzaman. Collectively, we have an extensive knowledge of data analysis fields and methodologies. We’ve even developed some of our own analytics tools! List of Services: We provide bioinformatics analysis support for life sciences and clinical & translational research for intramural investigators in NCI’s Division of Cancer Epidemi… [learn more]

The NCI Clinical Research Correlatives Core provides non-CLIA-certified spectral flow cytometric assays to support clinical trials conducted in the CCR. The core specializes in immunophenotyping and immune monitoring assays. Established Technologies Spectral flow cytometry (Cytek), Services offered include: Assistance with the experimental design, Assay development/panel optimization, Sample processing and preparation, Sample acquisition, Data analysis, Immunoprofiling assays offered include: “a la carte” CYTEK expandable antibody panel… [learn more]

The Center for Advanced Preclinical Research (CAPR) specializes in evaluating the efficacy of preclinical compounds, existing drugs, or biologics (therapeutics) in genetically engineered mouse (GEM) models, GEM-derived allograft (GDA) models, or patient-derived mouse xenografts (PDX). We partner primarily with Center for Cancer Research (CCR) investigators and clinicians using an RFA mechanism described below. Projects outside of CCR may be considered on a case-by-case basis. List of Services: One of the key features that distinguishes … [learn more]

The Crystallization Facility provides an automated environment for setting up crystallization experiments in a high-throughput format, storing the resulting plates under controlled conditions, and monitoring the status of prepared droplets remotely. The Facility is in Building 538, Rooms 215 and 222. The Facility also provides support in interpretation of results as well as collaborative efforts for all stages of determination of macromolecular structures using X-ray crystallography—from crystal growth, data collection, and structure solution to structure interpretation. [learn more]

The NMR Facility for Biological Research operates six NMR spectrometers with proton resonance frequencies from 850 to 500 MHz. Three of the highest field spectrometers (850, 700, 600) are equipped with higher sensitivity cryoprobes optimized for high-resolution multi-dimensional and relaxation NMR experiments applicable to small to large biological systems studied in solution. These high field instruments are dedicated to research projects conducted by laboratories within Center for Structural Biology.  The second 600 MHz spectrometer is set up as reconfigurable development system with multip… [learn more]

The primary objective of the Protein Technology Core (PTC) is to establish an experimental screening pipeline for single-domain antibodies termed nanobodies. Nanobodies are antibody fragments consisting of a single monomeric variable antibody domain. The PTC, which has recently undergone renovation, is equipped with state-of-the-art equipment to enable efficient screening of synthetic libraries utilizing ribosome display technologies and those derived from the immunization of alpacas. Enrichment of selection can be easily monitored by qPCR using QuantStudio5 for synthetic and immunogenic nano… [learn more]

The NCI Cloud Resources are components of the NCI Cancer Research Data Commons (CRDC) that bring data and computational power together to enable cancer research and discovery. These cloud-based platforms eliminate the need for researchers to download and store large data sets by allowing them to bring analysis tools to the data in the cloud, instead of the traditional process of bringing the data to the tools on local hardware. Cancer Research Data Commons (CRDC): Access harmonized, multi-omics cancer data – Data are available in nodes, harmonized and stored in a format that is re… [learn more]

The Mass Spectrometry Unit of the Collaborative Protein Technology Resource uses mass spectrometry for the study of proteins. We perform collaborative experiments with CCR researchers in areas such as: interactomes, quantitative global proteome analyses, and identification of sites of post-translational modification. In addition, we perform structural proteomics analyses. Finally, we also have an ICP-MS for analysis of inorganic atoms. Established Technologies: Mass Spectrometry Characterization of protein or RNA interactomes, Identification of proteins in complexes, org… [learn more]

Our operational objectives are to provide state-of-the-art OMICS technologies in support of the Genetics Branch (GB) investigators and collaborators. Research Services: Wet Lab Single cell isolation from fresh, frozen, and FFPE tissue, DNA/RNA extractions from fresh, frozen, FFPE tissue, and tissues preserved by other methods, Cell free circulating DNA from plasma and serum, Quality assessment of nucleic acids by spectrophotometry, fluorometry, qPCR, capillary electrophoresis, digital droplet PCR, Next-generation sequencing (NGS) library preps, … [learn more]

The NCI Genomic Data Commons (GDC) was established by the NCI Center for Cancer Genomics (CCG) to support the receipt, harmonization, distribution, and analysis of genomic and clinical data from cancer research programs. The GDC provides the cancer research community with a repository and computational platform for cancer researchers who need to understand cancer, its clinical progression, and response to therapy. The GDC accomplishes this by harmonizing raw sequence data against a common reference genome (Gr… [learn more]

The NCI High-Throughput Imaging Facility (HiTIF) works in a collaborative fashion with NCI/NIH Investigators by providing them with the necessary expertise, instrumentation, and software to develop and execute advanced High-Throughput Imaging (HTI) assays. These can be paired to screen libraries of RNAi or CRISPR/Cas9 reagents to discover and characterize novel cellular pathways by functional genomics. In addition to functional genomics screens, HiTIF also develops HTI assays to extract and quantify single-cell information about the molecular mechanisms underlying rare or heterogeneous b… [learn more]

The Medicinal Chemistry Accelerator (MCA) is a collaborative CCR resource that supports investigators in developing small molecule inhibitors for anticancer drug candidates. While CCR and NCATS have infrastructure to identify initial “hits” through high-throughput screening, advancing these hits into validated leads for preclinical studies requires specialized medicinal and synthetic chemistry expertise beyond the scope of individual sections. The MCA collaborates with researchers to design, synthesize, and optimize small molecules, improving potency and establishing structure-activity… [learn more]

The National Clinical Trials Network Biospecimen Banks (NCTN) receive, store, and distribute human cancer biospecimens collected on NCTN clinical trials. NCTN Biobanks provide cancer researchers with quality, well-annotated biospecimens and associated clinical information. The NCTN Biobanks house solid tumor biospecimens from all organ sites, as well as hematological malignancy specimens. Available Biospecimens Formalin-fixed, paraffin-embedded (FFPE) tissue, Fresh frozen tissue, Whole blood, serum, plasma, w… [learn more]

The National Cancer Institute (NCI) is developing a national repository of Patient-Derived Models (PDMs) comprised of patient-derived xenografts (PDXs), patient-derived organoids (PDOrg), and in vitro patient-derived tumor cell cultures (PDCs) and cancer-associated fibroblasts (CAFs). These models serve as a resource for public-private partnerships and for academic drug discovery efforts. These PDMs are clinically annotated with molecular information available in an easily accessible database available to the extramural community. List of Services: … [learn more]

Proteomic Data Common (PDC) represents the NCI’s largest public repository of proteogenomic comprehensive tumor datasets, essentially a Proteogenomic Cancer Atlas. It was developed to advance our understanding of how proteins help shape the risk, diagnosis, development, progression, and treatment of cancer. The objectives are (1) to make cancer-related proteomic data sets easily accessible to the public, and (2) facilitate multi-omic integration in support of precision medicine through interoperability with other resources. The PDC uses a cloud-based approach by storing all data sets with … [learn more]

The NCI Research Flow Facility provides cell sorting and benchtop flow cytometry services to NCI investigators. Services are program-specific and are not available to all NCI or NIH investigators. Please inquire as to availability. Established Technologies: Fluorescence-activated cell sorting (FACS), Benchtop polychromatic flow cytometry, Laser scanning cytometry, Full-spectrum cytometry, Developing Technologies: Stem cell analysis, Apoptosis detection, Integration of new laser technology into existing instrumentation, including solid-state diode and diode-pumpe… [learn more]

In order to meet increasing demands from both NIH intramural and extramural communities for access to a small angle X-ray scattering (SAXS) resource, the Center for Cancer Research (CCR) under the leadership of Drs. Jeffrey Strathern and Bob Wiltrout established a partnership user program (PUP) with the Argonne National Laboratory Photon Source in October 2008. This PUP agreement secures a certain percentage of the Argonne synchrotron beam time for biomedical research. In 2013, CCR established the CCR SAXS facility with Dr. Yun-Xing Wang as the facility head… [learn more]

The Specimen Resource Locator (SRL) is a biospecimen resource database designed to help researchers locate resources that may have the samples needed for their investigational use. This publicly searchable database includes information about biospecimen banks and sample procurement services. The specimens and samples come from non-commercial, either NCI or non-NCI-funded resources. Investigators can search the database and gain access to thousands of specimens of various tumor, organ, and preservation methods. Please submit all SRL inquiries using the “Contact the Expediter” button on t… [learn more]

The major goal of the Bioinformatics and Scientific Programming Core is to provide expertise and assistance in bioinformatics and computational analysis in support of genomic research being performed by NHGRI Investigators. Established Technologies: The work of the Core speaks directly to this goal by: providing access to specialized software and hardware, developing generalized software solutions that can address a variety of questions in genomic research, developing database solutions for the efficient archiving and retrieval of experimental and clinical data, disseminat… [learn more]

The Bioinformatics and Computational Bioscience Branch (BCBB) Science Support Team at the NIAID Office of Cyber Infrastructure and Computational Biology partners with researchers by applying bioinformatics and computational biology methods to analyze existing data and generate new hypotheses. The team offers expert assistance in areas such as Genomics, Transcriptomics, Phylogenetics, Computational Structural Biology, and Virtual and Augmented Reality, among others. Established Technologies: Genomics and Phylogenetics Comparative genomics, Metagenomics, Bulk and S… [learn more]

The Aerobiology Core specializes in particle generation and characterization with expertise in aerosols analysis, manufacturing, and customization. Work at the IRF is through collaborative projects with IRF scientists. Established Technologies Large- and small-particle generation and characterization, Sampler characterization, Plethysmography acquisition and analysis, Aerosol route of entry comparison, Exposure chamber characterization, Decontamination and operation of gloveboxes, BSL-4 operational (when approved), Aerosol manufacturing and customization to optimi… [learn more]

The Cell Culture Core provides high-quality mammalian cell cultures through routine quality control (QC) testing for Mycoplasma, endotoxin, and sterility and routine cell passage and viability monitoring. Cells are ordered through an online ordering system (SharePoint) and provided in a variety of standard flask and plate formats. Experienced cell culture staff is available for troubleshooting and training other staff. In addition, the Cell Culture Core can consult on new cell-based assay techniques and model system development. Established Technologies High-quality mammalian cell cul… [learn more]

The Clinical Core Services Laboratory provides a full-service core for immunology, clinical pathology, virology, high-containment training, and agent-specific assays required for monitoring animals in infectious disease studies. In addition, the Clinical Core Services Laboratory routinely performs assay optimization and ruggedization for high-throughput analysis of a wide variety of in vivo and in vitro studies involving high-consequence pathogens. Established Technologies Molecular biology, DNA/RNA high-throughput extraction, Validated quantitative PCR (qPCR) assays, DNA/protein microar… [learn more]

The IRF animal facility is staffed and operated currently by 14 contract personnel consisting of 1 veterinarian, 1 animal facility manager, 7 animal technicians, 5 cagewash and animal care personnel, and 1 animal program/IACUC coordinator.  Established TechnologiesAnimal facility maintenance, animal care, and husbandry, Animal acquisition, Animal study support, Maintaining the health of the animal colony, Supporting the animal regulatory program, Comparative Medicine (CM) personnel support multiple imaging sessions at NIHAC Poolesville, as well as imaging studies at NIH-Bethesda at Buildin… [learn more]

The Imaging Physics resource specializes in PET, CT, and MRI imaging modalities. Any work performed at the IRF is through collaboration with scientists in this facility. Established Technologies Standard radiographic projection images, Fluoroscopic image series, Planar radiographic studies, Computed tomography, Single Positron Emission Computed Tomography (SPECT), Positron Emission Tomography (PET), The diagnostic X-ray equipment available at the Integrated Research Facility (IRF) (MultiDiagnost Eleva, Philips Healthcare) can provide either standard radiographic p… [learn more]

The Pathology Core laboratories include two state-of-the-art BSL-4 necropsy suites and full-service histopathology and electron microscopy laboratories. We are equipped to perform complete high-containment/BSL-4 postmortem examinations on a variety of species, including rodents and nonhuman primates. Our fully digitized pathology core facility provides real-time gross pathology images from the postmortem table and gross tissue trimming stations using the MacroVIEW D imaging system.All work at the IRF is through collaborative projects with IRF scientists. Established Technologies: Gr… [learn more]

The NIDDK Regulatory Support Program provides regulatory support for all NIDDK-sponsored trials that require a U.S. or Canadian regulatory application for the use of an investigational drug or device in a clinical trial. Specifically, regulatory consultation is provided to aid in the determination of the need for a regulatory application, protocol development, study drug or device issues, and other regulatory matters. Regulatory support staff work with the program officials and data coordinating centers (or intramural investigators) to:initiate regulatory applications,, maintain applications (… [learn more]

The Office of Collaborative Biostatistics, Office of the Clinical Director, is the statistical and data management component of CCR. The Section provides statistical leadership and data management consultation for CCR’s major clinical activities and is involved in the design, review, conduct, monitoring, and analysis of intramural clinical trials of experimental treatments for cancer and AIDS-related malignancies. BDMS also provides support, on occasion, for national/multi-center clinical trials. The BDMS also provides a broad range of data analysis and consultation s… [learn more]

The Pan-Microbial Serology Facility (PMSF) is part of the Center for Cancer Research (CCR) at the National Cancer Institute (NCI). The PMSF focuses on determining individualized pan-microbial immune profiles associated with human diseases including immunological disorders such as cancer by providing a standardized intramural technological portal. Throughout our lives, we are exposed to various microbes including viruses, creating an individualized microbial landscape. Each microbe infection can leave unique immunological footprints, known as antigen-specific antibodies, which result in a pers… [learn more]

The Behavioral Health Core (BHC) is comprised of the two separate but interrelated components: 1) Psychosocial Support and Research Program, and 2) Health Psychology and Neurobehavioral Research Program. These teams provide specialized expertise, such as patient-reported and neurocognitive outcome assessments, to support NCI clinical trials and natural history studies. They also develop behavioral science protocols to investigate specific psychosocial and neurobehavioral effects of chronic illness. The BHC investigators develop novel assessment tool… [learn more]